题目

翻译一下这段学术英语
We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR. Dial-out PCR enables multiplex in vitro clone screening and is a compelling alternative to in vivo cloning and Sanger sequencing for accurate gene synthesis

提问时间：2021-01-07

答案

我们现在同一PCR、高度并行的方法获取准确的基因合成DNA分子.复杂的DNA分子库修改具有独特的侧面标签在大规模并行测序.Tag-directed引物然后使检索通过PCR分子所需的序列.同一体外克隆筛选和PCR使多路复用是一个引人注目的替代体内克隆和桑格测序对准确基因合成