题目

英语翻译
Bacillus subtilis natto strain was isolated from commercial natto available in the Australian market.Bacterial cells were cultivated in
a liquid culture constituting 0.5% (w/v) peptone,0.5% (w/v)
glucose and0.05%(w/v) yeast extract for five days before streaking
on tryptic soy agar plates for aseptic spore genesis.Plates were
scrapped after five days and harvested cells were suspended in a
0.9% (w/v) NaCl solution.The spore suspension was kept in a
heated water bath (808C,30 min) to kill the residual vegetative
cells,centrifugedat3000 rpm for 10 min to remove the cell debris,
diluted using 0.9% (w/v) NaCl solution to obtain the standard
spore solution (5.2 3 0.5 4 1010 spores/mL).

提问时间：2021-04-01

答案

纳豆芽孢杆菌菌株是从澳大利亚market.Bacterial细胞提供商业纳豆中分离培养在构成0.5％的液体培养物（重量/体积）蛋白胨,0.5％（重量/体积）划线前葡萄糖和0.05％（重量/体积）酵母提取物五天对胰蛋白酶大豆琼脂平板...