题目

英语翻译
The activity of the microsomal system as a function of [Pi] is shown in fig.2.The response is very complex >70% of the microsomal activity is inhibited at -5 mM.An increase in activity between 0.1-l mM was consistently observed.The steepest slope of this phosphate inhibition curve occurred overa [Pi] range which is roughly that reported in muscle in situ [ 141.
A change in activity of from 70 - 30% of the maximal rate occurred over -3-8 mM phosphate.Thus,insitu the content of free phosphate may be an important modifying factor in muscle tissue and the rate of hydrolysis of phosphate compounds in fish muscle after death may play an important role in the rate at which trimethylamine oxide is degraded.
The effect of several other compounds which were shown to be activators or inhibitors of DMA production by the microsomal system of red hake muscle are given in table 2.Phenazine methosulfate and methylene blue were the most potent activators of
the system.These are com

提问时间：2021-02-15

答案

microsomal 系统的活动作为功能[ Pi ] 被显示在fig.2 .反应是非常复杂>70% microsomal 活动被禁止在-5 毫米.在活动的增量在0.1-l 之间毫米一致地被观察了.这磷酸盐禁止曲线最陡峭的倾斜发生了大致是那被报告在肌肉里在原处的overa[ Pi ] 范围[ 141 .
一个变化在活动从70 上- 30% 最大的率发生了-3-8 毫米磷酸盐.因而,insitu 自由磷酸盐内容也许是一个重要修改因素在肌肉组织和磷酸盐化合物加水分解的率在鱼肌肉里在死亡也许充当在三甲胺氧化物被贬低的率之后的一个重要角色.
由红色无须鳕肌肉microsomal 系统证明是DMA 生产活化计或抗化剂几种其它化合物的作用被给在表2 .Phenazine methosulfate 和次甲基蓝色是最有力的活化计 系统.这些是可能参加电子调动反应并且次甲基蓝色频繁地被使用在分析用试样为这酵素对增加的活动的化合物.三甲胺和胆碱有相似的结构对基体三甲胺氧化物,能功能作为竞争抗化剂.
EDTA 和枸橼酸盐能可能禁止由结为螯合物Fe2+ 在反应媒介,并且Cu2+ 的禁止作用也许表明sulfhydryl 小组被介入了在酵素.trimethylaime 氧化物转换向DMA 由红色无须鳕肌肉microsomal 分数被审查了在冻结和未冻结的状态(表3) .DMA 生产在样品在温度结冻度以上被检验了在1 h 以后但是样品在冻结状态被检验了在3 h 以后.这些价值,然而,是全部被计算根据DMA yield/h 反应.