题目

关于像素和分辨率的问题（英文）
拜托认真作答 我会给很高的分的
You are working with this microscope configuration:
@ Objective:50X NA 0.85
@ Ocular:10X
@ Relay Lens:Adjustable from 0.1X to 1.0X in steps of 0.1X,set initially at 0.5X
@ Image Detector (CCD Array) of 1024 X 768 pixels spaced 13.2 μm apart
@ You are using a green filter in your system which provides illumination of about 530 nm wavelength
You also have available in your laboratory the following additional components which are compatible with your microscope:
@ Objectives:10X,NA 0.25,20X NA 0.5,100X Oil immersion,NA 1.3.
@ Oculars:1X,2.5X,5X,15X,20X
Questions:
a) What is the resolution of the objective lens,expressed in μm in the object plane (the stage of the microscope)?
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b) What is the separation of two features just at the resolution limit of the microscope when projected onto the ccd array?
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c) Using a factor of 2.3 for the

提问时间：2020-09-20

答案

您正在使用该显微镜配置工作：
@物镜：50倍 NA0.85
@目镜：10倍
@变焦镜头：0.1倍至1.0倍可调,初始调整值为每步0.1倍,可以设置为0.5倍
@图像检测器（CCD的阵列）为1024 x 768像素,间距13.2微米
@你是在你的系统上使用绿色滤镜提供约530纳米波长的照明
您也可以在您的实验室里使用下面这些与你的显微镜兼容的其它组件：
@物镜：10倍、NA 0.25,20倍、NA 0.5,100倍 油浸泡、NA 1.3.
@目镜：1倍,2.5倍,5倍,15倍,20倍
问题：